FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l01n01_nutrinetat_vega1_akita_r2y_ln.3510000008b2cc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l01n01_nutrinetat_vega1_akita_r2y_ln.3510000008b2cc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26289888
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC5172251.96739141680634TruSeq Adapter, Index 1 (100% over 51bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG378300.14389563013733644No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA273850.10416552554350934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC571950.042.65003645
ACGTCTG583100.042.2109815
CGTATGC579050.042.14916244
GCACACG584200.042.1083111
CACACGT591450.041.5579112
CTCGTAT590550.041.55375742
ACACGTC595550.041.30203213
AGCACAC596450.041.2925210
TCGTATG594650.041.07377243
CACGTCT604900.040.60039514
TCACGAT605150.040.44272635
CGTCTGA611650.040.3142616
GAGCACA617100.040.246019
TCGGAAG620500.040.1654173
TCTCGTA613750.040.0100641
CACGATC612200.039.8625436
CAGTCAC619600.039.6445627
ACGATCT619400.039.46470337
ATCGGAA633450.039.3833732
GATCGGA633400.039.368491
Produced by FastQC (version 0.11.2)