FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l01n01_nutrinetat_vega17_mh-1_r2y_ln.3510000008b65f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l01n01_nutrinetat_vega17_mh-1_r2y_ln.3510000008b65f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17762510
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC13079437.363503243629419TruSeq Adapter, Index 9 (100% over 51bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG318700.17942284057827412No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA200050.11262484862781218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC1387900.044.22556345
ACGTCTG1420000.043.92831415
GCACACG1419000.043.85304611
TCGGAAG1439550.043.6953353
AGCACAC1423950.043.67532310
CACACGT1424800.043.6729512
ACACGTC1427850.043.56863413
CGTATGC1410150.043.55573344
AGTCACG1418650.043.51387428
GATCGGA1448100.043.505371
CTCGTAT1421150.043.50153742
TCGTATG1412850.043.48009543
ATCGGAA1448900.043.4459842
CACGTCT1431150.043.4445914
GTCACGA1421150.043.42673529
GAGCACA1442650.043.4086159
CGATCAG1421600.043.3084133
TCACGAT1428500.043.2063230
ACGATCA1424600.043.17614732
CACGATC1425650.043.16329231