FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l01n01_nutrinetat_vega15_kn-0_r2y_ln.3510000008b5e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l01n01_nutrinetat_vega15_kn-0_r2y_ln.3510000008b5e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23891739
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC3703841.5502596943654876TruSeq Adapter, Index 8 (100% over 51bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG359230.1503574101491733No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA251020.10506560447525398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC405500.042.77827545
ACGTCTG418600.042.00265515
CGTATGC414450.041.89538244
GCACACG421650.041.70416611
CACACGT426850.041.17503412
CTCGTAT425850.041.09708442
AGCACAC429050.041.0320710
TCGTATG426550.040.7490543
ACACGTC433850.040.49513613
CACGTCT441700.039.7856314
GAGCACA449650.039.4973269
TCGGAAG450150.039.4813843
TCTCGTA445400.039.3364541
CGTCTGA452150.039.0004616
CTGAACT460250.038.4752419
CAGTCAC456450.038.46086527
GATCGGA463650.038.3462141
ATCGGAA464450.038.3045582
TCCAGTC467500.037.7633825
AATCTCG464650.037.7358339
Produced by FastQC (version 0.11.2)