FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l01n01_nutrinetat_vega13_ge-0_r2y_ln.3510000008b579.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l01n01_nutrinetat_vega13_ge-0_r2y_ln.3510000008b579.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16418276
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC2535721.5444496121273634TruSeq Adapter, Index 7 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191890.11687585225147877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC281600.042.09417345
GCACACG290650.042.04723711
ACGTCTG293300.041.88224415
CACACGT293550.041.62418712
CGTATGC286800.041.34967444
AGCACAC297500.041.0790910
ACACGTC298600.040.9277613
TCGTATG291050.040.73801843
CTCGTAT295000.040.45583342
CACGTCT303250.040.3150214
GAGCACA311600.039.5812579
TCGGAAG318850.039.000593
CGTCTGA315200.038.99369416
CAGTCAC308000.038.88258427
GATCGGA322650.038.5828821
CCAGATC309600.038.3192333
ATCGGAA324700.038.3048632
TCTCGTA312150.038.2962541
CAGATCA311800.038.12811334
AGAGCAC324350.038.0325138
Produced by FastQC (version 0.11.2)