FastQCFastQC Report
Mon 24 Apr 2017
C98KBACXX_l01n01_nutrinetat_vega11_edi-0_r2y_ln.3510000008b4f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC98KBACXX_l01n01_nutrinetat_vega11_edi-0_r2y_ln.3510000008b4f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20763790
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC2485421.1969972726559073TruSeq Adapter, Index 6 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT386850.186309917409105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC276250.042.70889329
TCACGCC280150.041.9776130
ACGTCTG294250.041.5485515
GTATGCC284400.041.52293845
CACGCCA282650.041.50284631
GCACACG296900.041.05636211
CGTATGC289700.040.7885544
CGCCAAT288650.040.7804533
CACACGT298600.040.77741212
ACGCCAA289450.040.56669232
AGCACAC301250.040.53072710
TATCTCG295150.040.37167439
AGTCACG293200.040.3546928
ACACGTC305650.039.85157413
TCGTATG298450.039.64529443
CTCGTAT301650.039.56340842
CACGTCT310200.039.24527714
GAGCACA322450.038.2635889
CAGTCAC312650.038.06729527
GCCAATA310600.037.94178834
Produced by FastQC (version 0.11.2)