FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l08n01_lib_h9.351000000a216b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l08n01_lib_h9.351000000a216b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8264376
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3529004.270134853496501No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220611.4769536139207606No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC752470.9104982638737636TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA122750.063.0841332
AGAGCAC130500.060.4902238
TCGGAAG129950.059.6620143
GATCGGA134600.057.6012271
CGGAAGA137150.057.255944
GAGCACA138500.057.0990879
GAAGAGC188950.041.7278376
TATGCCG94250.040.0932146-47
GCACACG98700.039.77318610-11
CGTATGC94950.039.72282444-45
AGTCACG97100.038.91713328-29
AAGAGCA203300.038.8992777
GTATGCC97050.038.7898744-45
ATGCCGT97950.038.65145546-47
ACGTCTG101650.038.5254714-15
GTCACGA97800.038.51715528-29
TGCCGTC100200.037.73589748-49
TCACGAT103150.036.58849330-31
CTCGTAT103450.036.43757242-43
CACGTCT109050.036.085414-15
Produced by FastQC (version 0.11.2)