FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l08n01_lib_h8.351000000a20e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l08n01_lib_h8.351000000a20e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17755522
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387270.21811242722123292No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA186290.10491947237597407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG49900.014.612746-47
GTATGCC53350.013.44496444-45
CGTATGC55100.013.36279344-45
CGGGCAT30100.012.3111461
AGAGCAC134250.011.7811198
TCGTATG64950.011.41013342-43
CCCGCAT24550.011.0304621
GAGCACA147100.011.0102799
CGGAAAT47650.010.9673111
CGGGGAT54050.010.8113471
CGCGAAT33250.010.7161791
GCACACG70800.010.56583810-11
ACGTCTG71150.010.54725514-15
ATGCCGT71200.010.17448746-47
CCGGGAT57700.010.0451031
CTCGTAT77550.09.648149542-43
CGGGAAT49900.09.6159281
CGGCAAT55300.09.3642231
ACACGTC81800.09.20306612-13
TCGGAAG159450.09.0874243
Produced by FastQC (version 0.11.2)