FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l08n01_lib_h7.351000000a2050.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l08n01_lib_h7.351000000a2050.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15707262
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2875411.8306245862582542No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1363340.8679679501112288No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC234460.14926853578936927TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA112250.022.6431242
TCGGAAG118150.021.3917753
AGAGCAC125750.020.7733298
TATGCCG58650.020.20888746-47
GAGCACA135850.019.6834019
ACGTCTG69250.019.066814-15
CGTATGC62950.018.82818844-45
GTATGCC64450.018.5742544-45
GCACACG71600.018.47411210-11
ACACGTC70600.018.33214212-13
GATCGGA145100.017.582361
CACACGT75450.017.37405612-13
CGGAAGA152000.016.970884
CACGTCT79400.016.68923614-15
CTCGTAT73350.016.28871342-43
TCGTATG76900.015.66031542-43
ATGCCGT76850.015.60836546-47
AGCACAC98750.013.92396410-11
CGTCTGA100600.013.26668716-17
TGCCGTC97250.012.67607848-49
Produced by FastQC (version 0.11.2)