FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l08n01_lib_h6.351000000a1fa4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l08n01_lib_h6.351000000a1fa4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16092968
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1106510.6875736035764193No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA433010.2690678313658487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC117250.013.529798
GCACACG58100.013.36592710-11
ATCGGAA113450.012.9812712
GTCACGC52900.012.75139228-29
GAGCACA128350.012.7297549
TATGCCG53600.012.31875546-47
GTATGCC55650.012.2915944-45
TCGGAAG120650.012.2459673
ACGTCTG66550.011.81160114-15
CGTATGC57300.011.77184444-45
AGTCACG61300.011.50776328-29
TCGTATG60950.011.45716942-43
CGGGAAT46950.011.3330421
CGGAAAT49100.010.9335461
CACACGT72900.010.88045512-13
CGCCAAT64750.010.63803132-33
CGGGGAT48750.010.5247831
GCCGAAT27250.010.4604131
AGCACAC79150.010.2913110-11
CCGGCAT35750.010.23242951
Produced by FastQC (version 0.11.2)