FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l08n01_lib_h5.351000000a1f10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l08n01_lib_h5.351000000a1f10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10571958
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380231.3055575892374902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA637130.6026603586582542No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC192500.18208547555712953TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG79500.024.1418383
ATCGGAA80450.023.7977072
TATGCCG40700.022.99307846-47
GAGCACA86950.022.8330679
AGAGCAC89950.021.9131328
GTATGCC43500.021.51275844-45
ACGTCTG47500.020.94830914-15
CGTATGC44750.020.75261744-45
GCACACG50700.019.62608110-11
CGGAAGA103250.019.0942154
GATCGGA101700.018.918831
ACACGTC52250.018.8620712-13
CACACGT53500.018.11064312-13
ATGCCGT53050.017.90894146-47
TCGTATG53500.017.80333342-43
CTCGTAT53600.017.77011742-43
CACGTCT56850.017.46121214-15
TGCCGTC61850.015.51428948-49
AGCACAC68100.015.16944410-11
CACAGTG62450.015.06171632-33
Produced by FastQC (version 0.11.2)