FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l08n01_lib_h4.351000000a1e8b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l08n01_lib_h4.351000000a1e8b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16610591
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1482150.8922921526392409No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA652080.3925688134756915No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC174890.1052882465169361TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA111300.018.6535662
TCGGAAG115250.018.0966913
AGAGCAC126550.017.1894238
TATGCCG55200.017.03910646-47
CGTATGC56600.016.78529744-45
GTATGCC58550.016.55078944-45
GAGCACA135900.016.531029
GCACACG66700.016.27115210-11
ACGTCTG66300.016.01117514-15
ACACGTC70550.015.51788412-13
CACACGT72750.014.82011112-13
ACTGACC70950.014.12719632-33
GATCGGA149000.014.0618881
TCGTATG71150.013.82063142-43
CACGTCT80950.013.26022314-15
CTCGTAT73550.013.17588942-43
ATGCCGT75400.013.07276546-47
CGGAAGA162950.013.0611754
CGCGAAT32250.012.6695171
CGCGTAT17500.012.4885231
Produced by FastQC (version 0.11.2)