FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l08n01_lib_h3.351000000a1e04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l08n01_lib_h3.351000000a1e04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18062540
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT917290.5078410899020847No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA433590.24004929539256384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG53100.012.43478646-47
GAGCACA119300.012.3816659
CGTATGC54900.012.02700444-45
ACTTAGG58500.011.73385832-33
ATCGGAA114750.011.5505332
TCGGAAG115200.011.5466523
AGAGCAC127450.011.5153678
GTATGCC58900.011.33120444-45
CACACGT62250.011.330440512-13
ACGTCTG64300.011.22781714-15
ACACGTC63600.011.16461612-13
CTCGTAT61050.010.9717142-43
CGCGTAT18900.010.80841351
GCACACG66750.010.60215410-11
CGGGGAT46350.010.3520631
TAGGCAT66400.010.33771134-35
CGCGAAT35650.010.2609241
CACTTAG67450.010.21203930-31
ATGCCGT69550.010.17670346-47
CCCATAA68150.010.105587
Produced by FastQC (version 0.11.2)