FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l08n01_lib_h2.351000000a1da6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l08n01_lib_h2.351000000a1da6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29044977
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT615540.2119264890449044No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC426640.14688942600987429TruSeq Adapter, Index 2 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA295830.10185237881235024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG105600.020.39994446-47
GTATGCC115600.018.57357244-45
CGTATGC116600.018.51612944-45
GAGCACA289050.016.1359989
CGATGTA137800.015.96122134-35
ATGCCGT141800.015.64430546-47
TCGTATG142500.015.36825342-43
AGAGCAC297300.015.224938
CTCGTAT147350.014.89464942-43
ACGTCTG159250.014.13696614-15
TATCTCG158650.014.07358638-39
CCGATGT157950.013.9700832-33
GCACACG168250.013.5501310-11
ACACGTC170450.013.50063112-13
TCGGAAG330900.013.3953123
GATGTAT175000.013.0705134-35
ATCGGAA338050.013.0698322
TGCCGTC172650.013.01390348-49
ATGTATC184100.012.43765236-37
CACGTCT186400.012.25621814-15
Produced by FastQC (version 0.11.2)