FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l08n01_lib_h11.351000000a225e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l08n01_lib_h11.351000000a225e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10972956
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT531490.4843635570943691No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC473930.4319073183196943TruSeq Adapter, Index 11 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA112710.10271616873338416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC124850.041.0852628
GAGCACA126950.040.667529
ATCGGAA123900.040.5667422
TCGGAAG128200.039.1690183
GATCGGA145400.034.568851
CGGAAGA153050.033.3043024
TATGCCG76850.032.32896446-47
ACGGCTA77900.031.86358832-33
CGTATGC79200.031.2793844-45
GTATGCC80700.030.78627844-45
GCACACG88450.029.26506610-11
TCGTATG85050.029.1576142-43
ACGTCTG89400.028.74164414-15
AGTCACG89350.028.0184528-29
ACACGTC92000.027.87769112-13
ATGCCGT89300.027.7685446-47
CACACGT92950.027.61831312-13
CGGCTAC89600.027.5967932-33
CACGTCT95550.026.8917114-15
CTCGTAT93600.026.62106342-43
Produced by FastQC (version 0.11.2)