FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l08n01_lib_h10.351000000a21d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l08n01_lib_h10.351000000a21d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9039782
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC233190.2579597605340483TruSeq Adapter, Index 10 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156590.17322320383389775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA89700.027.2229082
GAGCACA96000.026.2710029
TCGGAAG93900.026.1064593
AGAGCAC97900.025.8096588
TATGCCG48900.023.65472646-47
GATCGGA103650.023.605011
GTATGCC50700.023.51743544-45
CGTATGC50900.022.95839544-45
TCGTATG53250.022.43697242-43
TAGCTTA54200.022.087134-35
CGGAAGA112350.021.9028854
CTCGTAT54450.021.59350442-43
ACGTCTG60550.020.4730314-15
GCACACG61650.020.30022610-11
ACACGTC61050.020.11080212-13
TATCTCG59750.019.99673738-39
ACTAGCT62050.019.40767932-33
GTCACTA61750.019.38592728-29
ATGCCGT62350.019.27577646-47
CACTAGC63500.019.1883630-31
Produced by FastQC (version 0.11.2)