FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l08n01_lib_h1.351000000a1d56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l08n01_lib_h1.351000000a1d56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14394515
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354070.24597563724793786No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC151020.10491496240060884TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG45800.017.4243446-47
CGTATGC52600.015.48781244-45
GTATGCC53850.015.04008944-45
GAGCACA124550.013.8809399
AGAGCAC123900.013.6854188
TCGTATG62550.013.48038842-43
ATGCCGT66900.012.74532246-47
ACGTCTG66150.012.60114414-15
CTCGTAT64900.012.40670242-43
ATCGGAA126850.012.3967792
GCACACG70550.012.28646810-11
ACACGTC69050.012.03747912-13
TCGGAAG132250.011.9624423
CGGGAAT35800.011.9437271
CACACGT72150.011.2898712-13
CGGAAAT32700.011.187251
CACGTCT78450.010.68598214-15
CGAATTA36850.010.3139213
CGGGGAT43400.010.2900741
GTCACAT79250.010.21982928-29
Produced by FastQC (version 0.11.2)