FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l07n01_lib_g9.351000000a20a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l07n01_lib_g9.351000000a20a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7177813
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC102580.1429126114040586TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27100.022.17519846-47
CGTATGC29050.020.85056944-45
AGAGCAC62600.020.3324768
GTATGCC29650.019.8678544-45
GAGCACA66100.019.7588219
ATCGGAA64350.019.0462682
TCGGAAG68650.018.130073
ACGTCTG35100.017.79315614-15
GCACACG35850.017.68568610-11
AGTCACG33900.017.65448828-29
CTCGTAT36650.017.04486542-43
ATGCCGT36350.016.46692346-47
TCGTATG35550.016.36960642-43
ACACGTC38400.016.14020712-13
CACACGT39950.015.98951712-13
GTCACGA38550.015.03210328-29
CGGAAGA84650.014.8704594
CGTCTGA44900.014.70304816-17
CACGTCT45450.014.42047414-15
GATCGGA87700.014.0293931
Produced by FastQC (version 0.11.2)