FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l07n01_lib_g8.351000000a2029.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l07n01_lib_g8.351000000a2029.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9219598
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT482260.5230813751315405No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA169920.18430304661873545No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC161710.17539810304093517TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG37450.022.07352646-47
GAGCACA85000.021.9576269
AGAGCAC84400.021.6073028
CGTATGC39300.021.0346744-45
TCGGAAG84250.020.5813433
ATCGGAA84600.020.4400422
GTATGCC41150.020.08900544-45
ACGTCTG48000.018.74947714-15
GCACACG51500.017.75150910-11
TCGTATG47700.017.42863342-43
ACACGTC52600.017.24484812-13
ATGCCGT48550.017.07577946-47
CACACGT54200.016.73577512-13
CTCGTAT50200.016.70262342-43
CGGAAGA107150.016.669154
GATCGGA105650.016.592791
CACGTCT58450.015.55985114-15
TGCCGTC57500.014.91364248-49
GTCACAC58350.014.44907128-29
CGTCTGA66150.013.92866616-17
Produced by FastQC (version 0.11.2)