FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l07n01_lib_g7.351000000a1fb0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l07n01_lib_g7.351000000a1fb0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8000776
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT578030.722467420660196No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA271580.3394420741188105No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC233960.2924216351013952TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC87400.027.5506428
TATGCCG43750.026.71182446-47
CGTATGC43450.026.67792144-45
GAGCACA91700.026.4141149
GTATGCC47900.024.54661444-45
TCGGAAG97050.023.8886993
ATCGGAA97650.023.7419152
TCGTATG50650.023.11899842-43
CTCGTAT52300.022.38962442-43
ATGCCGT52800.022.26833546-47
ACGTCTG53850.022.09333614-15
CGGAAGA113500.021.0525654
GCACACG59800.020.33171310-11
CACACGT61000.019.6592412-13
ACACGTC61000.019.54245412-13
GATCGGA119700.019.4082161
CACGTCT63450.019.08744614-15
TGCCGTC63500.018.77787648-49
TCTCGTA66050.017.98071340-41
CGTCTGA69700.017.37636416-17
Produced by FastQC (version 0.11.2)