FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l07n01_lib_g6.351000000a1f03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l07n01_lib_g6.351000000a1f03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14641085
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT698860.47732801223406596No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA323920.22124043402521054No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC278210.19002007023386586TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG62150.022.28238946-47
CGTATGC66550.020.63063444-45
GTATGCC66900.020.59371644-45
GTCACGC68100.020.0177528-29
CGCCAAT73200.018.81972932-33
TCACGCC73250.018.80675530-31
GAGCACA161850.018.2516679
CTCGTAT77500.017.9298842-43
AGAGCAC166000.017.7953768
TCGTATG78600.017.5580742-43
ATGCCGT81250.017.5120846-47
AGTCACG79750.017.51044328-29
CACGCCA81600.017.02782630-31
ACGTCTG90050.016.19138314-15
TATCTCG87200.015.93584838-39
GCCAATA88750.015.89711334-35
TCGGAAG194300.014.64522653
TGCCGTC97300.014.62337348-49
CCAATAT97300.014.57342734-35
ACACGTC99950.014.53996912-13
Produced by FastQC (version 0.11.2)