FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l07n01_lib_g5.351000000a1e61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l07n01_lib_g5.351000000a1e61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6562199
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385220.5870288298175658No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA153970.234631714155575No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC93490.14246748688968439TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG24200.019.72995846-47
GAGCACA54700.018.5797469
CGTATGC26050.018.4202644-45
GTATGCC26150.018.25897844-45
AGAGCAC59800.017.0746028
TCGGAAG58550.016.2260343
ATCGGAA58600.016.1311282
ACGTCTG32350.015.48815714-15
TCGTATG31750.015.411942542-43
ATGCCGT32400.015.24978846-47
ACACGTC32550.015.17384612-13
GCACACG34150.015.15825210-11
CTCGTAT32200.015.0490242-43
CACACGT33500.014.88530712-13
GATCGGA70300.013.5144131
CGGAAGA74100.013.26884
CACGTCT38900.012.8192114-15
CGGGAAT18250.012.7542731
GTCACAC40000.012.29027328-29
CGCGAAT11950.011.9254811
Produced by FastQC (version 0.11.2)