FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l07n01_lib_g4.351000000a1ddc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l07n01_lib_g4.351000000a1ddc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15925562
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286450.17986806368277616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG45150.015.73043746-47
GTATGCC51400.014.23365144-45
CGTATGC52500.013.75444244-45
GAGCACA125450.012.5308269
TCGTATG65750.011.45187342-43
CGGGGAT43950.011.3495391
AGAGCAC140100.011.2204998
ATGCCGT65400.011.18665746-47
GCACACG67950.011.14791310-11
ACGTCTG68450.011.101294514-15
CTCGTAT70100.010.63958542-43
CACACGT72100.010.60505612-13
CGTTCTA49050.010.4111734-35
ACACGTC75100.010.213037512-13
ACTGACC70400.010.18900232-33
TCGGAAG139000.010.1504193
CCCGCAT26550.010.0200821
CCCATAA62450.09.8862898
ATCGGAA142300.09.7814912
CGGGCAT24300.09.7748821
Produced by FastQC (version 0.11.2)