FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l07n01_lib_g3.351000000a1d7f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l07n01_lib_g3.351000000a1d7f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16041431
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288060.1795725082132635No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC196550.12252647534998592TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG53150.018.36804646-47
CGTATGC56200.017.45603444-45
GTATGCC57450.016.82814444-45
ACTTAGG63550.015.62260132-33
CTCGTAT64350.015.46574742-43
TAGGCAT68200.014.31390634-35
TCGTATG68900.014.23758242-43
TTAGGCA69850.014.0437934-35
ATGCCGT71350.013.74929646-47
CACTTAG71700.013.74740930-31
AGAGCAC160900.012.9286358
GAGCACA162850.012.7446599
ACGTCTG81100.012.649466514-15
TCACTTA85000.011.70811630-31
CGTTCTA54900.011.59479734-35
CGGGAAT43450.011.37089351
CCCATAA64550.011.3307598
ACACGTC90200.011.29417912-13
CTTAGGC87650.011.24575932-33
CACGTCT95350.010.93334714-15
Produced by FastQC (version 0.11.2)