FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l07n01_lib_g2.351000000a1d49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l07n01_lib_g2.351000000a1d49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15413226
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT644700.41827713419630647No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC472410.3064965115025239TruSeq Adapter, Index 2 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA286240.18571063578773192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA183450.028.2446089
AGAGCAC182950.027.9324078
TATGCCG90800.027.0762846-47
CGTATGC91150.026.97248644-45
TCGGAAG190450.026.0668073
ATCGGAA191150.025.99622
GTATGCC96150.025.61927244-45
CGATGTA103050.023.99396534-35
TCGTATG104500.023.95705642-43
ATGCCGT104600.023.7084646-47
CTCGTAT107600.023.04609742-43
CGGAAGA224800.022.5047824
CCGATGT110650.022.3455432-33
ACGTCTG114700.022.3181114-15
GATCGGA229500.021.714771
GCACACG119350.021.6672610-11
TATCTCG118200.021.08034738-39
ACACGTC121300.021.04486712-13
CACACGT122600.020.84108412-13
GATGTAT123750.020.30671734-35
Produced by FastQC (version 0.11.2)