FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l07n01_lib_g12.351000000a2227.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l07n01_lib_g12.351000000a2227.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31469940
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAT86550.010.9231541
CGGGGAT89100.010.8771341
CGGGCAT51100.010.1336911
GGGAGAT277950.010.0501541
CGGGCCT32250.09.1332221
CGCCCAT59400.08.9576411
GGCAAAT176000.08.6377241
CTCGGAT141950.08.6012141
CGCGAAT69200.08.5815541
CCCGCAT48500.08.5219611
CGGGACT66850.08.3146981
CTGGAAT217550.08.1672261
GGGAAAT177450.08.1387851
GCCAGAT131250.08.1079441
GCGAATT82600.08.0520012
GGCAGAT143750.07.9977821
GGAAAAT229500.07.9696651
GGCGAAT98750.07.93795971
GTCAGAT192500.07.8973481
CCCGTAT27100.07.88869671
Produced by FastQC (version 0.11.2)