FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l07n01_lib_g11.351000000a21af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l07n01_lib_g11.351000000a21af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13750486
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217460.15814713749026763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCTA48100.015.94973632-33
CGTATGC46650.015.88612444-45
TATGCCG46550.015.86925246-47
GTATGCC48700.015.46127444-45
AGAGCAC103800.015.2363088
GCACACG54800.014.30000710-11
GAGCACA119800.013.9149979
TCGTATG55500.013.73786242-43
AGTCACG55650.013.61363728-29
ACGTCTG58050.013.33589614-15
ATCGGAA111950.013.2400052
TCGGAAG113900.013.0967513
ATGCCGT61050.012.80047346-47
CGGCTAC62200.012.715981532-33
GCTACAT60550.012.59191134-35
CTCGTAT60300.012.40795842-43
CGGGCAT19950.011.90673351
CACACGT66700.011.8911412-13
AGCACAC70350.011.67924710-11
ACACGTC72750.011.0328212-13
Produced by FastQC (version 0.11.2)