FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l07n01_lib_g10.351000000a211b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l07n01_lib_g10.351000000a211b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30973549
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1939080.6260438543868512No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA805260.2599831230189346No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC421020.1359288856436826TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG235400.020.2212723
ATCGGAA235600.020.0831242
AGAGCAC253400.020.0730638
TATGCCG107800.020.05191846-47
GAGCACA253600.019.9635949
CGTATGC115300.018.64477544-45
GTATGCC119750.018.2891444-45
GCACACG140800.017.91111810-11
ACGTCTG139900.017.89076814-15
GATCGGA284550.016.7620811
ACACGTC152600.016.24601212-13
TCGTATG135850.016.17312842-43
CGGAAGA304600.016.1252444
CACACGT157150.015.92674412-13
TAGCTTA137300.015.794435534-35
CTCGTAT140450.015.55886942-43
ATGCCGT149300.014.97142646-47
CACGTCT169800.014.75437514-15
ACTAGCT162700.013.63506532-33
TATCTCG163900.013.434607538-39
Produced by FastQC (version 0.11.2)