FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l07n01_lib_g1.351000000a1d2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l07n01_lib_g1.351000000a1d2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19403428
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347780.17923636998575715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT53050.011.4620451
CGGGCAT31150.011.1327481
CGCCCAT33950.010.7742851
GGGGAAT62150.010.548131
CGCGTAT21750.010.2654071
TATGCCG49500.010.07747846-47
CGGGAAT52550.09.763121
GGGAGAT171150.09.742441
CGGAAAT48250.09.6486441
CCGGGAT48850.09.627381
CGGGCCT19450.09.2811441
GCCAGAT79550.09.2561221
GTATGCC57500.09.04719744-45
CGGCAAT59400.09.0371091
GGAAAAT146650.09.0053431
GGCGAAT63150.08.9518161
CGTATGC54950.08.948297544-45
CGCAAAT44900.08.7815121
CCGGGTT55450.08.6528141
CGGGTAT33750.08.5860541
Produced by FastQC (version 0.11.2)