FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l06n01_lib_f9.351000000a21ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l06n01_lib_f9.351000000a21ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9617129
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560790.5831158134615851No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA196750.20458288539126385No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC119200.12394551430057765TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA73200.017.7124639
TATGCCG31600.017.21281846-47
AGAGCAC71900.016.976128
GCACACG38000.016.49834410-11
ATCGGAA71250.016.467412
GTATGCC34250.016.36668244-45
CGTATGC33650.015.952641544-45
TCGGAAG75350.015.6968063
ACGTCTG40200.015.3002814-15
AGTCACG38950.013.96473528-29
CACACGT44250.013.792412-13
TCGTATG41200.013.77860942-43
ACACGTC45850.013.67365512-13
ATGCCGT42800.013.42997546-47
CTCGTAT42950.012.83008442-43
GTCACGA44850.012.445436528-29
CGGGCAT16800.012.1586681
CACGTCT50550.012.12060314-15
GATCGGA99400.012.0431741
CGGAAGA101600.011.7346654
Produced by FastQC (version 0.11.2)