FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l06n01_lib_f8.351000000a2185.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l06n01_lib_f8.351000000a2185.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10084717
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350480.3475357811230598No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA125270.12421766520567705No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC106420.10552601525655107TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29950.018.23994346-47
GTATGCC34500.016.3855144-45
CGTATGC34700.015.81191944-45
GCACACG39150.015.16462710-11
AGAGCAC76900.015.1318198
ATCGGAA75200.014.9716672
GAGCACA81700.014.8240659
TCGGAAG79700.014.3639063
ACGTCTG40350.014.24294114-15
TCGTATG41250.013.70455242-43
ACACGTC43850.013.32253512-13
CGGGGAT25650.012.964981
CTCGTAT44350.012.58595242-43
CACACGT46100.012.56927612-13
ATGCCGT46200.012.13283946-47
CACGTCT49900.011.65986114-15
GATCGGA98950.011.4747521
CGGAAGA101250.011.4004174
CGCAAAT24150.011.0162071
GTCACAC52450.010.9585128-29
Produced by FastQC (version 0.11.2)