FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l06n01_lib_f7.351000000a210e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l06n01_lib_f7.351000000a210e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7335199
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT475650.6484486651282398No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA217920.29708805446178077No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC84630.1153751929565919TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG25100.018.92629446-47
AGAGCAC58550.017.3586778
GTATGCC27950.017.16644544-45
CGTATGC28300.016.70234344-45
ACGTCTG32100.015.61019214-15
GAGCACA66100.015.44780359
GCACACG32650.015.12835710-11
ATCGGAA62850.015.1164282
ATGCCGT33200.014.73799746-47
CGGGGAT22800.014.5850661
TCGGAAG66200.014.422743
TCGTATG33700.014.09643842-43
CTCGTAT34900.013.74786542-43
ACACGTC36550.013.57908512-13
CACACGT36700.013.20005312-13
CACGTCT38550.012.99837114-15
CGGAAAT18400.012.1345951
CGGAAGA80400.011.9932784
GATCGGA80600.011.9058821
CGGGCAT13250.011.4730611
Produced by FastQC (version 0.11.2)