FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l06n01_lib_f6.351000000a2093.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l06n01_lib_f6.351000000a2093.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9449345
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180460.19097619993766762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG25650.014.35267146-47
CGGGGAT27800.012.9873841
GTATGCC29850.012.65167644-45
AGAGCAC67900.012.5909678
CGTATGC30600.012.18634644-45
GAGCACA70650.011.8990659
GCACACG35200.011.80651510-11
GTCACGC32350.011.60010628-29
CCCGCAT16500.011.5167231
ACGTCTG37150.011.3147614-15
ATCGGAA70450.011.2611172
GGCGAAT35500.010.9733291
CACACGT37950.010.95094212-13
TCACGCC36450.010.94756130-31
TCGGAAG73400.010.80783753
ACACGTC38750.010.60228712-13
AGTCACG37000.010.59159728-29
CTCGTAT36150.010.57839442-43
CCGGAAT41000.010.5441211
ATGCCGT40200.010.3395246-47
Produced by FastQC (version 0.11.2)