FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l06n01_lib_f5.351000000a200f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l06n01_lib_f5.351000000a200f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12656141
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT605240.47821843957016597No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA279390.22075449380660347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT38500.012.5857051
TATGCCG32700.012.34818846-47
CGTATGC35500.011.70880744-45
CGGGAAT36050.011.4644181
GTATGCC37750.010.9480144-45
CGGGCAT20100.010.39907551
CCGGGAT34600.010.29729751
CGCGTAT12650.010.1393791
ATCGGAA87100.010.0895732
GAGCACA93150.010.044559
TCGTATG43150.09.908236542-43
CACGAAT31350.09.8494931
CGCCAAT39650.09.8244611
CTCGGAT61100.09.7964211
AGAGCAC93950.09.7569998
ACGTCTG46200.09.71509914-15
GCACACG46400.09.67305210-11
CGCGAAT28150.09.6191021
GGGGAAT42800.09.545371
TCGGAAG90700.09.5315323
Produced by FastQC (version 0.11.2)