FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l06n01_lib_f4.351000000a1f7d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l06n01_lib_f4.351000000a1f7d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12256052
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT575550.46960473078932763No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA253470.20681211208960276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG34250.014.84074446-47
GTATGCC38500.013.26438944-45
CGTATGC39300.012.93393844-45
CGGGGAT31450.012.6881991
AGAGCAC86600.012.4494978
ATCGGAA83700.012.372472
GAGCACA93750.011.8037889
ACGTCTG47850.011.76228714-15
TCGGAAG87800.011.7398413
ACACGTC48050.011.36706412-13
CTCGTAT45900.011.12608542-43
TCGTATG49200.010.91088542-43
GCACACG51400.010.81101810-11
ACTGACC48800.010.75765132-33
CGCCCAT23950.010.7109891
CGGGCGT10051.8189894E-1210.3991531
CGGGCAT17000.010.3393721
ATGCCGT51700.010.33699846-47
CGCGAAT24850.010.3230661
CACACGT52450.010.27765912-13
Produced by FastQC (version 0.11.2)