FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l06n01_lib_f3.351000000a1ee8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l06n01_lib_f3.351000000a1ee8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12110155
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136070.11236024642128858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29650.012.49687646-47
CGGGGAT35650.011.1932161
CGTATGC32600.010.92909444-45
CGCGAAT28850.010.867591
CGGGCAT18900.010.8079121
CGGGAAT32050.010.6718471
CGGAAAT28500.010.6676861
GTATGCC34950.010.53404244-45
CGTTCTA36150.010.38255334-35
CCCATAA44150.09.6818668
GAGCACA86700.09.6411779
ACCCATA47550.09.1894986
CGACAAT48750.09.0624051
GGCGAAT38450.09.0190681
ATGCCGT44700.08.92695446-47
GCGAATT29450.08.8714862
ACTTAGG41000.08.86456932-33
CTCGAAT62200.08.8593681
CGGCAAT39400.08.6810321
CCCTGCC45200.08.66919870-71
Produced by FastQC (version 0.11.2)