FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l06n01_lib_f2.351000000a1e98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l06n01_lib_f2.351000000a1e98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10598461
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330390.31173393948423267No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC204090.1925656942078666TruSeq Adapter, Index 2 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130280.12292350747905759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG45500.022.28992746-47
GAGCACA99150.021.7958749
CGTATGC48100.021.13469544-45
GTATGCC48500.021.10730644-45
TCGGAAG101200.020.7940083
AGAGCAC104000.020.7796258
ATCGGAA103000.020.4779932
ATGCCGT56300.018.7312546-47
CTCGTAT55050.018.63931342-43
CGATGTA55750.018.27901334-35
CACACGT58700.018.08419212-13
TCGTATG57900.017.88592342-43
ACGTCTG60250.017.57978814-15
GCACACG60950.017.57245810-11
CCGATGT59700.017.46716132-33
CGGAAGA123100.017.3260364
ACACGTC62700.017.04412312-13
TATCTCG61850.017.01331738-39
GATCGGA125500.016.6560941
ATGTATC63400.016.4105936-37
Produced by FastQC (version 0.11.2)