FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l06n01_lib_f12.351000000a2376.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l06n01_lib_f12.351000000a2376.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18169398
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT35700.011.0446691
TATGCCG46250.011.04135146-47
CGGGAAT47950.010.5017121
CGTATGC51350.010.03737544-45
CGGGCCT19750.09.8618771
GTATGCC53500.09.85598644-45
CGGGCAT31850.09.844131
CGGAAAT47200.09.7627581
CGCCCAT36650.09.2029521
CGGGGAT49450.09.1264131
CGCAAAT44050.08.9510711
CTGGGAT100650.08.8733351
CGACAAT79500.08.843771
GGAAAAC151100.08.8031161
GGGAGAT161950.08.6533421
CTCGCAT52850.08.629171
CTGGCAT64000.08.610351
CGGGGCT34900.08.4393521
CGCCAAT59150.08.4329081
CGAATTA31450.08.3071153