FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l06n01_lib_f11.351000000a2319.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l06n01_lib_f11.351000000a2319.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11454743
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT428910.37443878051214247No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC334500.29201877335877374TruSeq Adapter, Index 11 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140030.12224630443476558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC121100.029.1393268
GAGCACA122200.028.993249
ATCGGAA124250.027.7182352
TCGGAAG125750.027.6125663
TATGCCG61850.026.84349346-47
CGTATGC62950.026.44994444-45
GTATGCC65250.025.62681444-45
ACGGCTA65600.025.27620732-33
GATCGGA142150.024.3960151
CTCGTAT69950.023.83706942-43
TCGTATG70200.023.58300242-43
CGGAAGA155700.022.3923234
AGTCACG74350.022.36211628-29
ACGTCTG78200.022.22990414-15
ATGCCGT77050.022.01035746-47
CGGCTAC76600.021.80152732-33
GCACACG80350.021.75225810-11
ACACGTC81250.021.36517512-13
GCTACAT79600.020.950234-35
GGCTACA82400.020.52659434-35
Produced by FastQC (version 0.11.2)