FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l06n01_lib_f10.351000000a2277.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l06n01_lib_f10.351000000a2277.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15614746
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200310.7687028658679431No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA554380.355036194632945No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC206950.1325349768737833TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG49700.020.2153946-47
AGAGCAC119900.018.6178538
CGTATGC54550.018.54906744-45
GAGCACA127050.018.018299
GTATGCC55750.017.80896244-45
ATCGGAA121600.017.774822
TCGGAAG125400.017.2731883
ACGTCTG67550.016.5586214-15
GCACACG69150.016.24388510-11
ACACGTC69450.015.83170112-13
CACACGT72950.015.49531812-13
TAGCTTA65900.015.21178334-35
TCGTATG67650.014.9571342-43
ATGCCGT69900.014.61131846-47
CACGTCT77400.014.57408314-15
CTCGTAT72450.014.19567342-43
CGGAAGA157000.014.0384554
GATCGGA161950.013.5223311
ACTAGCT79700.012.93537132-33
TATCTCG81200.012.5204338-39
Produced by FastQC (version 0.11.2)