FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l06n01_lib_f1.351000000a1e2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l06n01_lib_f1.351000000a1e2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11126519
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC170040.1528240773237344TruSeq Adapter, Index 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149690.13453443974705837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG41500.019.57431646-47
CGTATGC42200.019.41882944-45
GTATGCC44800.018.55694444-45
GAGCACA106450.016.954349
AGAGCAC109250.016.7808788
TCGTATG49700.016.58374642-43
ACGTCTG55250.016.16184614-15
ATGCCGT52400.016.09183946-47
CTCGTAT55050.015.14465442-43
GCACACG62300.014.86624210-11
ACACGTC60150.014.76585812-13
TCGGAAG120250.014.6548883
ATCGGAA122900.014.3398632
CACACGT63250.014.2674312-13
CACGTCT67900.013.29074214-15
TGCCGTC65400.013.25652148-49
TCACGAT65450.012.70310734-35
CGGAAGA142250.012.3883484
GTCACAT67650.012.32328328-29
GATCGGA148350.011.9763531
Produced by FastQC (version 0.11.2)