FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l05n01_lib_e9.351000000a2291.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l05n01_lib_e9.351000000a2291.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9962666
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128710.12919232663224883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT16950.012.33387851
CGGGGAT29750.011.9781841
CGGGAAT27600.011.5340651
CGGGCCT9103.6379788E-1210.9646451
CGCGTAT10851.8189894E-1210.0719781
CGGAAAT26150.09.9932711
AATCTAA43100.09.2577945
CGCGAAT23650.09.2415191
CGGATAT30350.09.2365551
CGGCAAT28850.09.2227171
CTCGGAT51050.09.2141681
CGCCCTT20550.09.0171541
GTATGCC29450.08.95262144-45
CCCGCAT17000.08.943711
CGGGAGT24650.08.866611
TATGCCG28200.08.84412746-47
GGGAGAT90000.08.6580091
GTCGGAT43950.08.5405321
GGAAAAT74950.08.4947361
GCACACG31700.08.46555510-11
Produced by FastQC (version 0.11.2)