FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l05n01_lib_e8.351000000a221a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l05n01_lib_e8.351000000a221a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14365646
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5086303.540599566493564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3283552.285696027870936No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC605350.4213872456553642TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC169250.036.3976638
TCGGAAG169900.035.929953
TATGCCG85900.034.25905646-47
ATCGGAA180000.033.86112
CGTATGC87750.033.59103444-45
GAGCACA187250.033.1524779
GTATGCC89850.032.83237544-45
CGGAAGA190800.032.239284
TCGTATG94750.031.10878242-43
CTCGTAT96800.030.64626142-43
ATGCCGT98500.030.23838446-47
GATCGGA217000.028.1581231
ACGTCTG109300.028.0287714-15
GCACACG112750.027.27629910-11
GTCACAC114250.026.56690228-29
CACGTCT119500.025.79534114-15
TGCCGTC116250.025.60090848-49
ACACGTC121400.025.31324412-13
TCCAGTC127900.024.38218124-25
CACACGT128400.024.04421212-13
Produced by FastQC (version 0.11.2)