FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l05n01_lib_e7.351000000a2192.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l05n01_lib_e7.351000000a2192.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13662560
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410100.30016336616271033No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA199990.1463781311847853No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC187160.13698750453794897TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG52950.019.3331846-47
CGTATGC53750.019.13384244-45
GTATGCC54950.018.543144-45
CTCGTAT59800.017.2372142-43
AGAGCAC131100.016.484358
ATGCCGT63650.016.34434946-47
TCGTATG62850.016.3251442-43
GAGCACA144200.014.9868149
ACGTCTG71000.014.95144414-15
GCACACG75400.014.204905510-11
TCGGAAG152450.013.680353
ATCGGAA150100.013.6096782
TGCCGTC78550.013.33473448-49
CGGGCAT22950.012.836371
CACGTCT85150.012.52263314-15
CACACGT87450.012.30188212-13
TCTCGTA86550.011.99205640-41
CCAGTCA89750.011.987720526-27
CGGAAGA180500.011.8159554
CAGTCAC92550.011.77901926-27
Produced by FastQC (version 0.11.2)