FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l05n01_lib_e6.351000000a2128.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l05n01_lib_e6.351000000a2128.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14198301
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1092150.769211752870995No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600480.42292384138073985No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC161740.11391503814435264TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG47150.017.9338146-47
CGTATGC48400.017.8142344-45
GTATGCC51700.016.81497844-45
TCGTATG55750.015.93360942-43
GTCACGC55250.015.73328428-29
CGCCAAT57200.015.1136432-33
ATGCCGT61150.014.56595746-47
CTCGTAT60400.014.47099242-43
GAGCACA133950.014.0061849
AGTCACG63700.013.49707728-29
CACGCCA68250.013.36275830-31
AGAGCAC138650.013.3601148
TCACGCC67650.013.20041530-31
ACGTCTG68250.013.15301514-15
GCCAATA69100.012.68284334-35
TATCTCG69800.012.55598338-39
GCACACG73300.012.37641410-11
CGGGCAT26450.012.2149711
TCGGAAG151450.011.5116393
ATCGGAA158750.011.0720592
Produced by FastQC (version 0.11.2)