FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l05n01_lib_e5.351000000a2079.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l05n01_lib_e5.351000000a2079.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11680333
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338220.28956366226887537No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC173050.14815502263505673TruSeq Adapter, Index 5 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA154910.13262464349261274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG42100.019.91543846-47
CGTATGC44300.019.1408744-45
GTATGCC45800.018.82514644-45
GAGCACA103500.017.43859
AGAGCAC102150.017.2504888
CTCGTAT50400.017.01203342-43
TCGTATG54150.015.96549842-43
ATGCCGT55800.015.40889946-47
ACGTCTG56400.015.28492714-15
GCACACG59150.014.93564710-11
TCGGAAG119700.014.4861783
ATCGGAA120150.014.2342242
ACACGTC61650.014.06033312-13
CACACGT63600.013.62923912-13
CACGTCT66450.013.330614-15
CACAGTG67450.012.64063332-33
CGGAAGA143950.012.4071964
GTCACAC71050.012.30104328-29
TGCCGTC71350.012.28371248-49
CCCGTAT9400.012.132131
Produced by FastQC (version 0.11.2)