FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l05n01_lib_e4.351000000a1ff4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l05n01_lib_e4.351000000a1ff4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7846063
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267630.3411010082381444No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC150880.1923002657511162TruSeq Adapter, Index 4 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA114390.14579286452326473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33050.022.06321546-47
GAGCACA74300.021.5431549
CGTATGC35100.020.9776344-45
GTATGCC35450.020.7705244-45
AGAGCAC79100.020.1758128
TCGGAAG77250.019.9863413
ATCGGAA77350.019.8990882
ACTGACC39700.018.6647332-33
ACGTCTG42550.018.64168214-15
TCGTATG41100.018.20357742-43
GCACACG44050.018.00689110-11
ATGCCGT41950.017.7786846-47
CTCGTAT42100.017.71477342-43
ACACGTC44900.017.66600412-13
CGGAAGA95050.016.3916684
CACACGT48400.016.241312-13
CACGTCT50000.015.95906614-15
GATCGGA101050.015.4224161
AGCACAC54900.014.53466910-11
TCTCGTA52650.014.25531240-41
Produced by FastQC (version 0.11.2)