FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l05n01_lib_e3.351000000a1f8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l05n01_lib_e3.351000000a1f8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5774073
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83960.14540862230179633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG16450.012.85094346-47
CGCGTAT4454.7186404E-712.8109381
CGTATGC17300.012.21953844-45
GTATGCC18850.011.71878244-45
GAGCACA40850.011.6271589
CGGGGAT15450.011.3771351
CCCGCAT8701.8189894E-1110.9212021
GCACACG22400.010.81403610-11
CTCGTAT20300.010.64711242-43
CGTTCTA14900.010.5200534-35
CTCGAAT35750.010.49811
CGGGCAT8259.295036E-1010.3652131
AGAGCAC45500.010.3344998
CTGGAAT38350.010.2818771
ATGCCGT21550.010.25053646-47
CGGGCGT6101.8823594E-610.1244911
ACTTAGG20700.010.09649332-33
CGCAAAT13650.010.0931111
CGGAAAT15450.09.83968451
TCGGAAG45400.09.730563
Produced by FastQC (version 0.11.2)