FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l05n01_lib_e2.351000000a1f2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l05n01_lib_e2.351000000a1f2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12952291
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231330.17860160800896152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC41300.012.36488144-45
TATGCCG42000.012.10224746-47
GTATGCC43850.012.02499544-45
GAGCACA98000.011.3410329
AGAGCAC98750.011.1587028
CTCGGAT64700.010.794061
GCACACG51950.010.51417210-11
ATGCCGT53300.010.4277446-47
CGGGAAT36650.010.110961
ACGTCTG55050.010.09465114-15
TCGGAAG101900.010.0228793
ATCGGAA101650.09.9541022
CGATGTA51600.09.94194134-35
TCGTATG54800.09.925343542-43
CTGGAAT88800.09.6301111
CGGGCAT20900.09.5471721
CCGATGT55700.09.46587532-33
CGCGTAT12650.09.3890411
CTCGTAT54900.09.38810742-43
CTCGGTT76250.09.2213331
Produced by FastQC (version 0.11.2)