FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l05n01_lib_e12.351000000a2418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l05n01_lib_e12.351000000a2418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12985949
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG39650.015.575746-47
CGTATGC39950.015.1615144-45
GTATGCC44750.014.11912244-45
TCGTATG48250.012.99597742-43
AGAGCAC110650.011.7614168
CTCGTAT55300.011.4250742-43
ATGCCGT56000.011.40989546-47
GCACACG56450.011.35874410-11
GAGCACA122650.011.23028859
ACGTCTG56950.011.17563914-15
CGGGAAT34750.010.6645881
ACACGTC62000.010.57177412-13
TCGGAAG123050.010.1922423
ATCGGAA121100.010.0033042
CACACGT63450.09.99332712-13
TGCCGTC73600.09.48826848-49
GTCACCT67200.09.47176928-29
CTCGCAT34950.09.3800731
CACGTCT70100.09.04533314-15
AATCTAA59450.08.8687695
Produced by FastQC (version 0.11.2)