FastQCFastQC Report
Tue 24 Oct 2017
C8R14ACXX_l05n01_lib_e11.351000000a2390.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8R14ACXX_l05n01_lib_e11.351000000a2390.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12386314
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178070.14376351188900913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT42700.014.1320241
CGGCTAT36850.011.8625721
CGGGCAT18550.011.526491
CGGAAAT37900.010.5309751
CCCGGAT31100.010.3890791
CGGGAAT33600.010.3231471
CCGGGAT35300.010.2298061
GCACACG36800.010.06732310-11
CGTATGC36750.09.8238444-45
GGGAGAT96750.09.674841
GGAAATT59450.09.6707921
GTATGCC38550.09.6115944-45
TATGCCG37550.09.42478446-47
ACGGCTA37450.09.38573332-33
CGCACAT21500.09.2819531
GGAAAAT112850.09.1366441
AGAGCAC83850.09.1198988
GGGACAT48650.09.0829861
CTCGAAT53750.08.9283541
CGGGGTT31550.08.8854861
Produced by FastQC (version 0.11.2)